ELISA/assay

Rat IL-1ra (Interleukin 1 Receptor Antagonist) ELISA Kit

MBS2505494

24-Strip-Wells

215 USD

ELISA Kit

Rat IL-1ra (Interleukin 1 Receptor Antagonist) ELISA Kit

[IL-1ra]

[interleukin-1 receptor antagonist protein isoform 3; Interleukin-1 receptor antagonist protein; interleukin-1 receptor antagonist protein; IL1 inhibitor; IL1RN (IL1F3); intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); type II interleukin-1 receptor antagonist; interleukin 1 receptor antagonist; ICIL-1RA; IL1 inhibitor; INN: Anakinra]

[IL-1ra]

[IL1RN; IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA; IL1F3; IL1RA; IL-1RN; IL-1ra; IRAP]

IL1RA_HUMAN

Rat

159

This kit recognizes Rat IL-10 in samples. No significant cross-reactivity or interference between Rat IL-10 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-10 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-10 were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IL-10 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IL-10. You can calculate the concentration of Rat IL-10 in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene [1]. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signaling via the phosphorylation of the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and Tyk2 respectively [2]. Interleukin 10 (IL-10) is a potent anti-inflammatory cytokine that plays a crucial, and often essential, role in preventing inflammatory and autoimmune pathologies. Deficiency or aberrant expression of IL-10 can enhance inflammatory response to microbial challenge but also lead to development of inflammatory bowel disease and a number of autoimmune diseases. Thus impaired IL-10 expression or signaling can enhance clearance of pathogens during an acute infection, but also exaggerate inflammatory response, resulting in exacerbated immunopathology and tissue damage. Conversely, some pathogens can harness the immunosuppressive capacity of IL-10 to limit host immune response, leading to persistent infection. All in all, Il-10 plays a largely nonredundant role in mediating host anti-inflammatory response and, therefore, identifying the cellular sources of IL-10 as well as the molecular mechanisms that regulate IL-10 expression are critical to developing therapeutic strategies directed against pathology-associated impaired IL-10 production [3].

10835147

NP_000568.1

NM_000577.4

P18510

16,142 Da

Cytokine Signaling In Immune System Pathway (366171); IL-1 Signaling Pathway (198908); IL1-mediated Signaling Events Pathway (137944); Immune System Pathway (106386); Interleukin-1 Signaling Pathway (160141); Leptin Signaling Pathway (672456); SIDS Susceptibility Pathways (198901); Signaling By Interleukins Pathway (160140)

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Vesicle; Secreted, signal peptide; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 2q14.2. Cellular Component: extracellular space; cytoplasm; plasma membrane; intracellular. Molecular Function: interleukin-1 Type I receptor antagonist activity; interleukin-1 receptor binding; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type II receptor binding; cytokine activity; interleukin-1, Type I receptor binding; interleukin-1 receptor antagonist activity. Biological Process: response to drug; positive regulation of JNK activity; negative regulation of heterotypic cell-cell adhesion; negative regulation of glutamate secretion; response to glucocorticoid stimulus; carboxylic acid metabolic process; response to lipopolysaccharide; female pregnancy; sensory perception of pain; fever; chronic inflammatory response to antigenic stimulus; memory; response to starvation; insulin secretion; acute-phase response; immune response; negative regulation of cytokine and chemokine mediated signaling pathway; response to activity; lipid metabolic process; negative regulation of membrane potential; negative regulation of cell migration; negative regulation of apoptosis. Disease: Gastric Cancer, Hereditary Diffuse; Microvascular Complications Of Diabetes, Susceptibility To, 4; Osteomyelitis, Sterile Multifocal, With Periostitis And Pustulosis

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590