ELISA/assay

Human TGF-alpha (Transforming Growth Factor alpha) ELISA Kit

MBS2505418

24-Strip-Wells

215 USD

ELISA Kit

Human TGF-alpha (Transforming Growth Factor alpha) ELISA Kit

[TGF-alpha]

[TGF-alpha, partial; TGF-alpha; TGF-alpha]

[TGF-alpha]

[tgfa]

D7RNZ2_GRYBI

Human

173

This kit recognizes Human TF in samples. No significant cross-reactivity or interference between Human TF and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Urine. Please Consult Technical Support For The Applicability If Other Biological Fluids Need To Be Tested. Assay Type: Quantitative Sandwich. Detection Range: 0.14-100ng/Ml. Sensitivity: 0.13ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TF were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TF were tested on 3 different plates,20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TF concentrations in serum, plasma, urine. Please consult technical support for the applicability if other biological fluids need to be tested. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TF. Samples (or Standards) and biotinylated detection antibody specific for Human TF are added to the micro ELISA plate wells. Human TF would combined with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Human TF. You can calculate the concentration of Human TF in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Transferrin is an iron binding protein found in blood plasma that is critical to cell health. Transferrin supplies iron to cells naturally in the body and as a supplement in cell culture media Transferrin (Tf) carries ferric iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body [1]. It shares homologous amino acid sequences with four other proteins: lactotransferrin, ovotransferrin, melanoma antigen p97, and HuBlym-1. Antigen p97 and the Tf receptor genes have been mapped on human chromosome 3. Transferrin has a molecular weight of around 80 kDa and contains two specific high-affinity Fe (III) binding sites. The affinity of transferrin for Fe (III) is extremely high (association constant is 1020M-1 at pH 7.4) but decreases progressively with decreasing pH below neutrality [2]. When not bound to iron, transferrin is known as Apo transferrin.

297499581

ADI43984.1

19,279 Da

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590