Human DKK2 (Dickkopf Related Protein 2) ELISA Kit | MyBioSource MBS2505377 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human DKK2 (Dickkopf Related Protein 2) ELISA Kit

catalog :

MBS2505377

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2505377

products type :

ELISA Kit

products full name :

Human DKK2 (Dickkopf Related Protein 2) ELISA Kit

products short name :

DKK2

other names :

dickkopf-related protein 2; Dickkopf-related protein 2; dickkopf-related protein 2; dickkopf 2 homolog; dickkopf homolog 2; dickkopf related protein-2; dickkopf-2; hDkk-2; dickkopf WNT signaling pathway inhibitor 2

products gene name :

DKK2

other gene names :

DKK2; DKK2; DKK-2; Dickkopf-2; Dkk-2; hDkk-2

uniprot entry name :

DKK2_HUMAN

reactivity :

Human

sequence length :

259

specificity :

This kit recognizes natural and recombinant Human DKK2. No significant cross-reactivity or interference between Human DKK2 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Human DKK2 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human DKK2 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DKK2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DKK2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DKK2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of DKK2. You can calculate the concentration of DKK2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

7657023

ncbi acc num :

NP_055236.1

ncbi gb acc num :

NM_014421.2

uniprot acc num :

Q9UBU2

ncbi mol weight :

28,447 Da

ncbi pathways :

Disease Pathway (530764); Presenilin Action In Notch And Wnt Signaling Pathway (137914); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1127632); Signal Transduction Pathway (477114); Signaling By WNT In Cancer Pathway (1127614); Signaling By Wnt Pathway (106510); TCF Dependent Signaling In Response To WNT Pathway (1127516); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471); XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway (1127633)

ncbi summary :

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008]

uniprot summary :

DKK2: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP5 and LRP6. Expressed in heart, brain, skeletal muscle and lung. Belongs to the dickkopf family. Protein type: Inhibitor; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q25. Cellular Component: extracellular space. Biological Process: Wnt receptor signaling pathway; multicellular organismal development

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage