ELISA/assay

Human ApoA1 (Apolipoprotein A1) ELISA Kit

MBS2505357

24-Strip-Wells

190 USD

ELISA Kit

Human ApoA1 (Apolipoprotein A1) ELISA Kit

[ApoA1]

[apolipoprotein A-I preproprotein; Apolipoprotein A-I; apolipoprotein A-I; apo-AI; apolipoprotein A-I; Apolipoprotein A1Cleaved into the following 2 chains:Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-IAlternative name(s):Apolipoprotein A-I(1-242)]

[ApoA1]

[APOA1; APOA1; Apo-AI; ApoA-I; ProapoA-I]

APOA1_HUMAN

Human

267

This kit recognizes Human ANG1 in samples. No significant cross-reactivity or interference between Human ANG1 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Detection Range: 78.13-5000pg/mL. Sensitivity: 46.88pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ANG1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ANG1 were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ANG1 concentrations in serum, plasma and other biological fluids!!Background/Introduction: Angiotensin is a peptide hormone that causes vasoconstriction and an increase in blood pressure. It is part of the renin-angiotensin system, which regulates blood pressure. Angiotensin also stimulates the release of aldosterone from the adrenal cortex to promote sodium retention by the kidneys. An oligopeptide, angiotensin is a hormone and a dipsogen. It is derived from the precursor molecule angiotensinogen, a serum globulin produced in the liver. Angiotensin was isolated in the late 1930s (first named 'angiotonin' or 'hypertensin') and subsequently characterized and synthesized by groups at the Cleveland in Clinic and Ciba laboratories [1].The reninangiotensin-aldosterone system regulates renal vasomotor activity, maintains optimal salt and water homeostasis, and controls tissue growth in the kidney. However, pathologic consequences can result from overactivity of this cascade, involving it in the pathophysiology of kidney disease [2].

4557321

NP_000030.1

NM_000039.1

P02647

30,778 Da

ABC-family Proteins Mediated Transport Pathway (106573); ABCA Transporters In Lipid Homeostasis Pathway (477112); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Amyloids Pathway (366238); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911)

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family. Protein type: Secreted, signal peptide; Lipid-binding; Secreted; Cell development/differentiation; Motility/polarity/chemotaxis; Endoplasmic reticulum; Vesicle. Chromosomal Location of Human Ortholog: 11q23-q24. Cellular Component: extracellular space; chylomicron; cell surface; endocytic vesicle; endoplasmic reticulum lumen; early endosome; extracellular region; plasma membrane; cytoplasmic vesicle; cytosol; nucleus; vesicle. Molecular Function: identical protein binding; lipase inhibitor activity; beta-amyloid binding; cholesterol binding; phosphatidylcholine binding; high-density lipoprotein binding; protein binding; enzyme binding; phospholipid transporter activity; cholesterol transporter activity; chemorepellent activity; phospholipid binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding. Biological Process: phototransduction, visible light; negative chemotaxis; negative regulation of lipase activity; axon regeneration in the peripheral nervous system; sequestering of lipid; negative regulation of interleukin-1 beta secretion; regulation of cholesterol absorption; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; response to drug; platelet activation; cholesterol metabolic process; organ regeneration; regulation of Cdc42 protein signal transduction; adrenal gland development; positive regulation of hydrolase activity; positive regulation of Rho protein signal transduction; lipoprotein metabolic process; positive regulation of transferase activity; vitamin transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; response to estrogen stimulus; lipoprotein biosynthetic process; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; blood vessel endothelial cell migration; cellular lipid metabolic process; platelet degranulation; phospholipid efflux; retinoid metabolic process; transmembrane transport; response to nutrient; phospholipid homeostasis; integrin-mediated signaling pathway; positive regulation of fatty acid biosynthetic process; receptor-mediated endocytosis; regulation of protein amino acid phosphorylation; cholesterol transport; negative regulation of heterotypic cell-cell adhesion; protein amino acid oxidation; protein stabilization; neurite regeneration; cholesterol efflux; glucocorticoid metabolic process; G-protein coupled receptor protein signaling pathway; reverse cholesterol transport; endothelial cell proliferation; negative regulation of inflammatory response; blood coagulation. Disease: Hypoalphalipoproteinemia, Primary; Amyloidosis, Familial Visceral

24-Strip-Wells

190 USD

48-Strip-Wells

310

96-Strip-Wells

355

5x96-Strip-Wells

1435

10x96-Strip-Wells

2805