ELISA/assay

Mouse GLUT1 (Glucose Transporter 1) ELISA Kit

MBS2505196

24-Strip-Wells

215 USD

ELISA Kit

Mouse GLUT1 (Glucose Transporter 1) ELISA Kit

[GLUT1]

[glucose transporter-like protein I, partial; Solute carrier family 2, facilitated glucose transporter member 1; solute carrier family 2, facilitated glucose transporter member 1; glucose transporter type 1, erythrocyte/brain; hepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter]

[GLUT1]

[SLC2A1; SLC2A1; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; GLUT1DS; GLUT1; GLUT-1]

GTR1_HUMAN

Mouse

130

This kit recognizes natural and some recombinant Mouse GLUT1. No significant crossreactivity or interference between Mouse GLUT1 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse GLUT1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Mouse GLUT1. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Mouse GLUT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Mouse GLUT1, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GLUT1. The concentration of Mouse GLUT1 in samples can be calculated by comparing the OD of the samples with the standard curve.

14268550

AAK56795.1

P11166

54,084 Da

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001)

This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]

GLUT1: an integral membrane protein that plays an important role in the glycolytic pathway by serving as a uniporter for glucose. One of 13 members of the human equilibrative glucose transport protein family. Transports a wide range of aldoses, including both pentoses and hexoses, and dehydroascorbic acid. Shown to transport water against an osmotic gradient. A receptor for the Human T-cell Leukemia virus (HTLV). Plays a role in the constitutive or basal uptake of glucose. Expressed at highest levels in proliferating cells of the early developing embryo, cells forming the blood tissue barriers, in human erythrocytes, astrocytes and in cardiac muscle. GLUT1 and GLUT3 are both essential for normal embryonic development. Is practically the only member of the GLUT family expressed on human red blood cells, where it comprises 10 - 20% of the integral membrane protein content. Several glycolytic proteins including the transporters GLUT1 and GLUT3, as well as multiple enzymes including HK2, PFKL, LDHA, ALDOA, ALDOC, PGK1, ENO1, PKM2, CA9 and PFKFB3 are induced in cancer cells by HIF-1 alpha. Polyps from Peutz-Jeghers patients exhibit up-regulated mTORC1 signaling, HIF-1alpha, and GLUT1 levels. Defects in GLUT1 are the cause of autosomal dominant GLUT1 deficiency syndrome, a blood-brain barrier glucose transport defect characterized by infantile seizures, delayed development, and acquired microcephaly. Defects also cause dystonia type 18, an exercise-induced paroxysmal dystonia/dyskinesia. Cytochalasin B binds to its inner surface, inhibiting its glucose transport activity with an IC50 of 0.44 uM. Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 1p34.2. Cellular Component: cortical actin cytoskeleton; membrane; integral to plasma membrane; basolateral plasma membrane; plasma membrane; melanosome; female pronucleus; caveola; midbody; intercellular junction; cytosol. Molecular Function: xenobiotic transporter activity; identical protein binding; D-glucose transmembrane transporter activity; protein binding; protein self-association; dehydroascorbic acid transporter activity; glucose transmembrane transporter activity; kinase binding. Biological Process: vitamin metabolic process; L-ascorbic acid metabolic process; pathogenesis; glucose transport; response to osmotic stress; cellular response to glucose starvation; hexose transport; carbohydrate metabolic process; energy reserve metabolic process; protein complex assembly; xenobiotic transport; regulation of insulin secretion; transmembrane transport; water-soluble vitamin metabolic process. Disease: Epilepsy, Idiopathic Generalized, Susceptibility To, 12; Dystonia 9; Glut1 Deficiency Syndrome 1; Glut1 Deficiency Syndrome 2

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590