Human PKM2 (Pyruvate Kinase, Muscle) ELISA Kit | MyBioSource MBS2505089 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PKM2 (Pyruvate Kinase, Muscle) ELISA Kit

catalog :

MBS2505089

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2505089

products type :

ELISA Kit

products full name :

Human PKM2 (Pyruvate Kinase, Muscle) ELISA Kit

products short name :

PKM2

other names :

pyruvate kinase PKM isoform f; Pyruvate kinase PKM; pyruvate kinase PKM; OIP-3; OPA-interacting protein 3; PK, muscle type; cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; p58; pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tumor M2-PK; pyruvate kinase, muscle; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58

products gene name :

PKM2

other gene names :

PKM; PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30; OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1

uniprot entry name :

KPYM_HUMAN

reactivity :

Human

sequence length :

536

specificity :

This kit recognizes natural and recombinant Human PKM2. No significant cross-reactivity or interference between Human PKM2 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: 46.875pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PKM2 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PKM2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PKM2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PKM2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PKM2. You can calculate the concentration of PKM2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

332164781

ncbi acc num :

NP_001193728.1

ncbi gb acc num :

NM_001206799.1

uniprot acc num :

P14618

ncbi mol weight :

56,273 Da

ncbi pathways :

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Disease Pathway (530764); Glucose Metabolism Pathway (106199)

ncbi summary :

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

uniprot summary :

PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported. Protein type: Carbohydrate Metabolism - pyruvate; Kinase, other; EC 2.7.1.40; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Nucleotide Metabolism - purine; Mitochondrial. Chromosomal Location of Human Ortholog: 15q22. Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol; vesicle; cilium. Molecular Function: potassium ion binding; protein binding; magnesium ion binding; pyruvate kinase activity; ATP binding. Biological Process: glycolysis; carbohydrate metabolic process; programmed cell death; glucose metabolic process; pathogenesis; phosphorylation

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage