catalog number :
MBS2505074
products type :
ELISA Kit
products full name :
Human PTH (Parathyroid Hormone) ELISA Kit
products short name :
[PTH]
other names :
[pth; Peptidyl-tRNA hydrolase]
products gene name :
[PTH]
other gene names :
[pth; ]
uniprot entry name :
PTH_STACT
specificity :
This kit recognizes natural and recombinantHumanPTH. No significant cross-reactivity or interference between HumanPTH and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanPTHconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PTH. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PTHand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PTH.You can calculate the concentration of PTHin the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
CAL27065.1
ncbi mol weight :
21,544 Da
uniprot summary :
The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.
size5 :
10x96-Strip-Wells