ELISA/assay

Mouse 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit

MBS2505038

24-Strip-Wells

215 USD

ELISA Kit

Mouse 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit

[15-LO]

[15-LO]

Mouse

This kit recognizes natural and some recombinant Mouse 15-LO. No significant crossreactivity or interference between Mouse 15-LO and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse 15-LO concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Mouse 15-LO. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Mouse 15-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Mouse 15-LO, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse 15-LO. The concentration of Mouse 15-LO in samples can be calculated by comparing the OD of the samples with the standard curve.

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590