Mouse C5a (Complement Component 5a) ELISA Kit | MyBioSource MBS2505021 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse C5a (Complement Component 5a) ELISA Kit

catalog :

MBS2505021

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2505021

products type :

ELISA Kit

products full name :

Mouse C5a (Complement Component 5a) ELISA Kit

products short name :

[C5a]

other names :

[C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin receptor; C5a ligand; C5a-R; complement component 5 receptor 1; complement component 5a receptor 1; C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD_antigen: CD88]

products gene name :

[C5a]

other gene names :

[C5AR1; C5AR1; C5A; C5AR; C5R1; CD88; C5AR; C5R1; C5a-R; C5aR]

uniprot entry name :

C5AR1_HUMAN

reactivity :

Mouse

sequence length :

350

specificity :

This kit recognizes MouseACTHin samples. No significantcross-reactivity or interference between MouseACTH and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level MouseACTH were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level MouseACTH were testedon 3 different plates, 20 replicates in eachplate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of MouseACTH concentrations in ser um, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses theCompetitive-ELISA principle. The micro ELISA plate provided in this kit has beenpre-coated with MouseACTH. During the reaction, MouseACTH in the sample or standard competes with a fixed amount of MouseACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MouseACTH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MouseACTH in the samples is then determined by comparing the OD of the samples to the standard curve.

ncbi gi num :

4502509

ncbi acc num :

NP_001727.1

ncbi gb acc num :

NM_001736.3

uniprot acc num :

P21730

ncbi mol weight :

39,336 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637)

uniprot summary :

C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia. Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: cell surface; apical part of cell; basolateral plasma membrane; integral to plasma membrane; cytoplasmic membrane-bound vesicle; plasma membrane. Molecular Function: complement component C5a binding; complement component C5a receptor activity; C5a anaphylatoxin receptor activity. Biological Process: neutrophil chemotaxis; organ regeneration; sensory perception of chemical stimulus; apoptosis; activation of MAPK activity; response to lipopolysaccharide; cell proliferation in hindbrain; signal transduction; chemotaxis; mRNA transcription from RNA polymerase II promoter; elevation of cytosolic calcium ion concentration; defense response to Gram-positive bacterium; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590