catalog number :
MBS2504956
products type :
ELISA Kit
products full name :
Mouse IL-4 (Interleukin 4) ELISA Kit
products short name :
IL-4
other names :
interleukin-4; Interleukin-4; interleukin-4; B-cell stimulatory factor 1; BSF-1; IL-4; lymphocyte stimulatory factor 1; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1
products gene name :
IL-4
other gene names :
IL4; IL4; IL-4; BSF-1
uniprot entry name :
IL4_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse IL-4. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse IL-4 and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL-4 in Mouse serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse IL-4. You can calculate the concentration of Mouse IL-4 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_999288.1
ncbi gb acc num :
NM_214123.1
ncbi mol weight :
15,023 Da
ncbi pathways :
Allograft Rejection Pathway (84530); Allograft Rejection Pathway (535); Asthma Pathway (84527); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (84528); Autoimmune Thyroid Disease Pathway (533); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Fc Epsilon RI Signaling Pathway (84494); Fc Epsilon RI Signaling Pathway (493)