Human PDPN (Podoplanin) ELISA Kit | MyBioSource MBS2504939 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PDPN (Podoplanin) ELISA Kit

catalog :

MBS2504939

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2504939

products type :

ELISA Kit

products full name :

Human PDPN (Podoplanin) ELISA Kit

products short name :

PDPN

other names :

podoplanin isoform d; Podoplanin; podoplanin; PA2.26 antigen; PDPN; T1-alpha; glycoprotein 36; glycoprotein, 36-KD; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); podoplanin; Aggrus; Glycoprotein 36; Gp36; PA2.26 antigen; T1-alpha; T1A

products gene name :

PDPN

other gene names :

PDPN; PDPN; T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26; Gp36; T1A

uniprot entry name :

PDPN_HUMAN

reactivity :

Human

sequence length :

118

specificity :

This kit recognizes natural and recombinant Human PDPN. No significant cross-reactivity or interference between Human PDPN and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Human PDPN is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PDPN concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDPN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDPN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PDPN. You can calculate the concentration of PDPN in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

54792062

ncbi acc num :

NP_001006626.1

ncbi gb acc num :

NM_001006625.1

uniprot acc num :

Q86YL7

ncbi mol weight :

12,421 Da

ncbi summary :

This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

PDPN: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. Belongs to the podoplanin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1p36.21. Cellular Component: ruffle; filopodium membrane; integral to plasma membrane; microvillus membrane; lamellipodium; apical plasma membrane; plasma membrane; filopodium; external side of plasma membrane. Molecular Function: folic acid transporter activity; water transporter activity; water channel activity; amino acid transmembrane transporter activity. Biological Process: nervous system development; water transport; cell morphogenesis; positive regulation of cell motility; signal transduction; prostaglandin metabolic process; folic acid transport; cell proliferation; regulation of cell shape; cell-cell adhesion; response to hyperoxia; amino acid transport; lymphangiogenesis; inflammatory response; tube morphogenesis; alveolus development; positive regulation of cell migration; lung development

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage