catalog number :
MBS2504918
products type :
ELISA Kit
products full name :
Mouse Cr (Creatinine) ELISA Kit
other names :
calretinin; Calretinin; calretinin; CR; calbindin 2
other gene names :
Calb2; Calb2; CR
uniprot entry name :
CALB2_RAT
specificity :
This kit recognizes natural and recombinant Mouse Cr. No significant cross-reactivity or interference between Mouse Cr and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 3.125-200mug/mL. Sensitivity: The minimum detectable dose of Mouse Cr is 1.875mug/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse Cr concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Cr. During the reaction, Cr in the sample or standard competes with a fixed amount of Cr on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cr. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cr in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_446440.1
ncbi gb acc num :
NM_053988.1
ncbi mol weight :
31,405 Da
ncbi summary :
displays increased mRNA expression following trimethyltin-induced neurodegeneration [RGD, Feb 2006]
uniprot summary :
CALB2: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Belongs to the calbindin family. Protein type: Calcium-binding. Cellular Component: cytoplasm; gap junction; terminal button; nucleus. Molecular Function: calcium ion binding. Biological Process: cytosolic calcium ion homeostasis