Human NRIP1 (Nuclear Receptor Interacting Protein 1) ELISA Kit | MyBioSource MBS2504911 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human NRIP1 (Nuclear Receptor Interacting Protein 1) ELISA Kit

catalog :

MBS2504911

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2504911

products type :

ELISA Kit

products full name :

Human NRIP1 (Nuclear Receptor Interacting Protein 1) ELISA Kit

products short name :

[NRIP1]

other names :

[nuclear receptor-interacting protein 1; Nuclear receptor-interacting protein 1; nuclear receptor-interacting protein 1; nuclear factor RIP140; receptor interacting protein 140; receptor-interacting protein 140; nuclear receptor interacting protein 1; Nuclear factor RIP140; Receptor-interacting protein 140]

products gene name :

[NRIP1]

other gene names :

[NRIP1; NRIP1; RIP140]

uniprot entry name :

NRIP1_HUMAN

reactivity :

Human

sequence length :

1158

specificity :

This kit recognizes Human NPTX1 in samples. No Significant cross-reactivity or interference between Human NPTX1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10 ng/mL. Sensitivity: 0.10 ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human NPTX1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human NPTX1 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human NPTX1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NPTX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NPTX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NPTX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human NPTX1. You can calculate the concentration of Human NPTX1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

57232746

ncbi acc num :

NP_003480.2

ncbi gb acc num :

NM_003489.3

uniprot acc num :

P48552

ncbi mol weight :

126,942 Da

ncbi pathways :

Adipogenesis Pathway (198832); Coregulation Of Androgen Receptor Activity Pathway (138085); FOXA1 Transcription Factor Network Pathway (137979); Ovarian Infertility Genes Pathway (198801); Retinoic Acid Receptors-mediated Signaling Pathway (138039)

ncbi summary :

Nuclear receptor interacting protein 1 (NRIP1) is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor. [provided by RefSeq, Jul 2008]

uniprot summary :

RIP140: Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. Interacts with RARA and RXRB homodimers and RARA/RXRB heterodimers in the presence of ligand. Interacts with HDAC1 and HDAC3 via its N-terminal domain. Interacts with NR2C1 (sumoylated form and via the ligand-binding domain); the interaction results in promoting the repressor activity of NR2C1. Interacts with CTBP1, CTBP2, ESR1, HDAC1, HDAC2, HDAC5, HDAC6, NR2C2, NR3C1, NR3C2, YWHAH, JUN and FOS. Found in a complex with both NR3C1 and YWHAH. Interacts with ZNF366. Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 21q11.2. Cellular Component: nucleoplasm; histone deacetylase complex; nucleolus; nuclear speck; cell junction; nucleus. Molecular Function: retinoid X receptor binding; protein binding; androgen receptor binding; histone deacetylase binding; transcription coactivator activity; estrogen receptor binding; transcription corepressor activity; nuclear hormone receptor binding; glucocorticoid receptor binding. Biological Process: circadian rhythm; sequestering of lipid; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; negative regulation of transcription from RNA polymerase II promoter; ovarian follicle rupture

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590