Rat IL-21 (Interleukin 21) ELISA Kit | MyBioSource MBS2504873 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat IL-21 (Interleukin 21) ELISA Kit

catalog :

MBS2504873

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2504873

products type :

ELISA Kit

products full name :

Rat IL-21 (Interleukin 21) ELISA Kit

products short name :

[IL-21]

other names :

[interleukin-21 isoform 2; Interleukin-21; interleukin-21; interleukin-21 isoform; interleukin 21; Za11]

products gene name :

[IL-21]

other gene names :

[IL21; IL21; Za11; IL-21; CVID11; IL-21]

uniprot entry name :

IL21_HUMAN

reactivity :

Rat

sequence length :

153

specificity :

This kit recognizes natural and recombinant Rat IL-21. No significant cross-reactivity or interference between Rat IL-21 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IL-21 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-21. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-21 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-21. You can calculate the concentration of IL-21 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

333033767

ncbi acc num :

NP_001193935.1

ncbi gb acc num :

NM_001207006.2

uniprot acc num :

Q9HBE4

ncbi mol weight :

16,867 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)

ncbi summary :

This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

uniprot summary :

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Apoptosis; Secreted, signal peptide; Cell development/differentiation; Cytokine. Chromosomal Location of Human Ortholog: 4q26-q27. Cellular Component: extracellular space. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity. Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; signal transduction; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tissue remodeling; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of cell proliferation; positive regulation of B cell proliferation; immune response; positive regulation of T cell proliferation; positive regulation of interferon-gamma biosynthetic process; positive regulation of inflammatory response. Disease: Immunodeficiency, Common Variable, 11

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590