ELISA/assay

Human PAH (Phenylalanine Hydroxylase) ELISA Kit

MBS2504801

24-Strip-Wells

215 USD

ELISA Kit

Human PAH (Phenylalanine Hydroxylase) ELISA Kit

[PAH]

[PAH; Phenylalanine-4-hydroxylase; phenylalanine-4-hydroxylase; phe-4-monooxygenase; phenylalanine 4-monooxygenase; phenylalanine hydroxylase; Phe-4-monooxygenase]

[PAH]

[PAH; PAH; PH; PKU; PKU1; PAH]

PH4H_HUMAN

Human

19

This kit recognizes Human PACAP-38 in samples. No Significant cross-reactivity or interference between Human PACAP-38 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20 ng/mL. Sensitivity: 0.19 ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PACAP-38 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PACAP-38 were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PACAP-38 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PACAP-38. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PACAP-38 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PACAP-38, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PACAP-38. You can calculate the concentration of Human PACAP-38 in the samples by comparing the OD of the samples to the standard curve.

1009166

AAA80910.1

P00439

51,862 Da

Abnormal Metabolism In Phenylketonuria Pathway (645287); Biogenic Amine Synthesis Pathway (198793); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Disease Pathway (530764); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Phenylalanine And Tyrosine Catabolism Pathway (106189); Phenylalanine Metabolism Pathway (82960)

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Jul 2008]

PAH: phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. Protein type: Oxidoreductase; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; EC 1.14.16.1. Chromosomal Location of Human Ortholog: 12q22-q24.2. Cellular Component: cytosol. Molecular Function: amino acid binding; iron ion binding; phenylalanine 4-monooxygenase activity. Biological Process: L-phenylalanine catabolic process; catecholamine biosynthetic process; amino acid biosynthetic process; neurotransmitter biosynthetic process. Disease: Phenylketonuria

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590