ELISA/assay

Human CD59 (Protectin) ELISA Kit

MBS2504754

48-Strip-Wells

410 USD

ELISA Kit

Human CD59 (Protectin) ELISA Kit

CD59

CD59 glycoprotein preproprotein; CD59 glycoprotein; CD59 glycoprotein; 1F5 antigen; 20 kDa homologous restriction factor; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); Ly-6-like protein; MEM43 antigen; T cell-activating protein; human leukocyte antigen MIC11; lymphocytic antigen CD59/MEM43; membrane attack complex (MAC) inhibition factor; membrane attack complex inhibition factor; membrane inhibitor of reactive lysis; protectin; surface anitgen recognized by monoclonal antibody 16.3A5; CD59 molecule, complement regulatory protein; 1F5 antigen; 20 kDa homologous restriction factor; HRF-20; HRF20; MAC-inhibitory protein; MAC-IP; MEM43 antigen; Membrane attack complex inhibition factor; MACIF; Membrane inhibitor of reactive lysis; MIRL; Protectin; CD_antigen: CD59

CD59

CD59; CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20; MIC11; MIN1; MIN2; MIN3; MSK21; HRF-20; HRF20; MAC-IP; MACIF; MIRL

CD59_HUMAN

Human

128

This assay has high sensitivity and excellent specificity for detection of Human CD59. No significant cross-reactivity or interference between Human CD59 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CD59 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25--2000pg/mL. Sensitivity: 18.75pg/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD59 in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD59. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CD59 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD59, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CD59. You can calculate the concentration of Human CD59 in the samples by comparing the OD of the samples to the standard curve.

42716299

NP_976076.1

NM_203331.2

P13987

14,177 Da

Arf6 Trafficking Events Pathway (137954); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (106386); Innate Immune System Pathway (106387); Regulation Of Complement Cascade Pathway (576254)

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D). Protein type: Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 11p13. Cellular Component: anchored to external side of plasma membrane; extracellular space; compact myelin; cell surface; focal adhesion; membrane; plasma membrane; extracellular region; vesicle; sarcolemma. Molecular Function: complement binding; protein binding. Biological Process: cell activation; cell surface receptor linked signal transduction; regulation of complement activation; negative regulation of activation of membrane attack complex; innate immune response; positive regulation of T cell proliferation; blood coagulation; negative regulation of apoptosis. Disease: Hemolytic Anemia, Cd59-mediated, With Or Without Immune-mediated Polyneuropathy

48-Strip-Wells

410 USD

96-Strip-Wells

490