ELISA/assay

Human MUC4 (Mucin 4) ELISA Kit

MBS2504728

96-Strip-Wells

445 USD

ELISA Kit

Human MUC4 (Mucin 4) ELISA Kit

MUC4

mucin-4 isoform e; Mucin-4; mucin-4; ascites sialoglycoprotein; mucin 4, tracheobronchial; pancreatic adenocarcinoma mucin; testis mucin; tracheobronchial mucin; mucin 4, cell surface associated; Ascites sialoglycoprotein; ASGP; Pancreatic adenocarcinoma mucin; Testis mucin; Tracheobronchial mucinCleaved into the following 2 chains:Mucin-4 alpha chainAlternative name(s):Ascites sialoglycoprotein 1; ASGP-1

MUC4

MUC4; MUC4; ASGP; MUC-4; HSA276359; MUC-4; ASGP; ASGP-1; ASGP-2

MUC4_HUMAN

Human

1125

This kit recognizes natural and recombinant Human MUC4. No significant cross-reactivity or interference between Human MUC4 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: The minimum detectable dose of Human MUC4 is 0.188ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MUC4 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MUC4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MUC4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MUC4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MUC4. You can calculate the concentration of MUC4 in the samples by comparing the OD of the samples to the standard curve.

112382231

NP_612154.2

NM_138297.4

Q99102

206,605 Da

Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (1127679); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number ( 100) of 48 nt tandem repeats. [provided by RefSeq, Jul 2008]

MUC4: May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 Tyr-1248 . In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. 14 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q29. Cellular Component: proteinaceous extracellular matrix; extracellular space; membrane; integral to plasma membrane; Golgi lumen; vesicle. Molecular Function: ErbB-2 class receptor binding; extracellular matrix constituent, lubricant activity. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; maintenance of gastrointestinal epithelium; cell-matrix adhesion; post-translational protein modification

96-Strip-Wells

445 USD