ELISA/assay

Rabbit TLR9 (Toll-Like Receptor 9) ELISA Kit

MBS2504726

48-Strip-Wells

450 USD

ELISA Kit

Rabbit TLR9 (Toll-Like Receptor 9) ELISA Kit

TLR9

TLR9; Toll-like receptor 9; toll-like receptor 9; toll-like receptor 9; CD_antigen: CD289

TLR9/CD289

TLR9; TLR9; CD289

TLR9_HUMAN

Rabbit

1032

This assay has high sensitivity and excellent specificity for detection of Rabbit TLR9/CD289. No significant cross-reactivity or interference between Rabbit TLR9/CD289 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rabbit TLR9/CD289 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TLR9/CD289 in Rabbit serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit TLR9/CD289. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rabbit TLR9/CD289 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit TLR9/CD289, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rabbit TLR9/CD289. You can calculate the concentration of Rabbit TLR9/CD289 in the samples by comparing the OD of the samples to the standard curve.

74054321

AAZ95520.1

Q9NR96

114,237 Da

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); IGF1R Signaling Cascade Pathway (730346); IRS-mediated Signalling Pathway (106426); IRS-related Events Pathway (106424); IRS-related Events Triggered By IGF1R Pathway (730348)

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Jul 2008]

TLR9: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Interacts with MYD88 via their respective TIR domains. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with BTK. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. Belongs to the Toll-like receptor family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: Golgi membrane; endoplasmic reticulum membrane; basolateral plasma membrane; endoplasmic reticulum; lysosome; cytoplasm; apical plasma membrane; integral to membrane; extracellular region; plasma membrane; endosome membrane; endosome. Molecular Function: siRNA binding; transmembrane receptor activity; interleukin-1 receptor binding. Biological Process: positive regulation of JNK activity; regulation of cytokine secretion; positive regulation of interleukin-12 production; maintenance of gastrointestinal epithelium; positive regulation of JNK cascade; positive regulation of interferon-alpha biosynthetic process; positive regulation of NF-kappaB import into nucleus; defense response to Gram-negative bacterium; positive regulation of interleukin-18 production; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to molecule of bacterial origin; negative regulation of interleukin-6 production; positive regulation of interferon-beta production; negative regulation of interleukin-8 production; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; defense response to bacterium; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; insulin receptor signaling pathway; negative regulation of toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; positive regulation of inflammatory response; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of interferon-gamma biosynthetic process

48-Strip-Wells

450 USD

96-Strip-Wells

535