Human FTL (Ferritin, Light Polypeptide) ELISA Kit | MyBioSource MBS2504610 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human FTL (Ferritin, Light Polypeptide) ELISA Kit

catalog :

MBS2504610

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2504610

products type :

ELISA Kit

products full name :

Human FTL (Ferritin, Light Polypeptide) ELISA Kit

products short name :

FTL

other names :

ferritin light chain; Ferritin light chain; ferritin light chain; ferritin L subunit; ferritin L-chain; ferritin light polypeptide-like 3; ferritin, light polypeptide

products gene name :

FTL

other gene names :

FTL; FTL; LFTD; NBIA3; Ferritin L subunit

uniprot entry name :

FRIL_HUMAN

reactivity :

Human

sequence length :

175

specificity :

This assay has high sensitivity and excellent specificity for detection of Human FTL. No significant cross-reactivity or interference between Human FTL and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human FTL and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 3.125--200ng/mL. Sensitivity: 1.875ng/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FTL in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FTL. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human FTL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FTL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FTL. You can calculate the concentration of Human FTL in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

20149498

ncbi acc num :

NP_000137.2

ncbi gb acc num :

NM_000146.3

uniprot acc num :

P02792

ncbi mol weight :

20,020 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Clathrin Derived Vesicle Budding Pathway (119545); Golgi Associated Vesicle Biogenesis Pathway (119546); Integrated Pancreatic Cancer Pathway (711360); Iron Uptake And Transport Pathway (187191); Membrane Trafficking Pathway (106160); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Scavenging By Class A Receptors Pathway (833814); Transmembrane Transport Of Small Molecules Pathway (106572)

ncbi summary :

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

uniprot summary :

FTL: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS). It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene. Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3); also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. Belongs to the ferritin family. Protein type: Oxidoreductase. Chromosomal Location of Human Ortholog: 19q13.33. Cellular Component: ferritin complex; membrane; cytosol. Molecular Function: identical protein binding; protein binding; ferric iron binding; iron ion binding. Biological Process: receptor-mediated endocytosis; iron ion homeostasis; cellular iron ion homeostasis; post-Golgi vesicle-mediated transport; transmembrane transport; iron ion transport. Disease: Hyperferritinemia With Or Without Cataract; L-ferritin Deficiency; Neurodegeneration With Brain Iron Accumulation 3

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage