ELISA/assay

Human Ntn1(Netrin 1)ELISA Kit

MBS2504380

48-Strip-Wells

410 USD

ELISA Kit

Human Ntn1(Netrin 1)ELISA Kit

Ntn1

netrin-1; Netrin-1; netrin-1; epididymis tissue protein Li 131P; netrin 1, mouse, homolog of; netrin 1; Epididymis tissue protein Li 131P

Ntn1

NTN1; NTN1; NTN1L; NTN1L

NET1_HUMAN

Human

604

This kit recognizes natural and recombinant Human Ntn1. No significant cross-reactivity or interference between Human Ntn1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: The minimum detectable dose of Human Ntn1 is 18.75pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human Ntn1 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Ntn1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Ntn1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Ntn1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Ntn1. You can calculate the concentration of Ntn1 in the samples by comparing the OD of the samples to the standard curve.

148613884

NP_004813.2

NM_004822.2

O95631

67,748 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Cell-Cell Communication Pathway (477132); DCC Mediated Attractive Signaling Pathway (161000); DSCAM Interactions Pathway (187229); Developmental Biology Pathway (477129); Netrin Mediated Repulsion Signals Pathway (161001); Netrin-1 Signaling Pathway (160999)

Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008]

NTN1: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: cytoplasm; extracellular region; basement membrane. Molecular Function: protein binding. Biological Process: axon guidance; inner ear morphogenesis; cell-cell adhesion; establishment of nucleus localization; apoptosis; positive regulation of axon extension; positive regulation of cell proliferation; neuron migration; negative regulation of axon extension; regulation of cell migration; anterior/posterior axon guidance

48-Strip-Wells

410 USD

96-Strip-Wells

490