Rat TIMP-2(Tissue Inhibitors of Metalloproteinase 2)ELISA Kit | MyBioSource MBS2504314 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat TIMP-2(Tissue Inhibitors of Metalloproteinase 2)ELISA Kit

catalog :

MBS2504314

quantity :

96-Strip-Wells

price :

445 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2504314

products type :

ELISA Kit

products full name :

Rat TIMP-2(Tissue Inhibitors of Metalloproteinase 2)ELISA Kit

products short name :

TIMP-2

other names :

tissue inhibitor of metalloproteinase type 2; Metalloproteinase inhibitor 2; metalloproteinase inhibitor 2; TIMP-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2; TIMP metallopeptidase inhibitor 2; Tissue inhibitor of metalloproteinases 2; TIMP-2

products gene name :

TIMP-2

other gene names :

Timp2; Timp2; Timp-2; TIMP-2

uniprot entry name :

TIMP2_RAT

reactivity :

Rat

sequence length :

220

specificity :

This kit recognizes natural and recombinant Rat TIMP-2. No significant cross-reactivity or interference between Rat TIMP-2 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: 0.375ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat TIMP-2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TIMP-2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TIMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TIMP-2. You can calculate the concentration of TIMP-2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

619233

ncbi acc num :

AAC60687.1

uniprot acc num :

P30121

ncbi mol weight :

24,356 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1121495); Degradation Of The Extracellular Matrix Pathway (1121494); Extracellular Matrix Organization Pathway (1121481); Matrix Metalloproteinases Pathway (198492)

ncbi summary :

acts as an inhibitor of metallopeptidase activity; may play a role in tissue remodeling during spermatogenesis [RGD, Feb 2006]

uniprot summary :

TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Extracellular matrix; Inhibitor; Cell development/differentiation. Cellular Component: extracellular space; proteinaceous extracellular matrix; neuron projection; growth cone; cell surface; cell soma; basement membrane; nucleus. Molecular Function: integrin binding; metalloendopeptidase inhibitor activity; protease binding; metal ion binding; enzyme activator activity. Biological Process: response to drug; negative regulation of proteolysis; positive regulation of catalytic activity; regulation of Rap protein signal transduction; negative regulation of metalloenzyme activity; central nervous system development; regulation of neuron differentiation; regulation of cAMP metabolic process; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to cytokine stimulus; regulation of MAPKKK cascade; negative regulation of Ras protein signal transduction; spermatogenesis; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging

size1 :

96-Strip-Wells

price1 :

445 USD

more info or order :

MyBioSource product webpage