Human NE/ELA2 (Elastase 2, Neutrophil) ELISA Kit | MyBioSource MBS2504270 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human NE/ELA2 (Elastase 2, Neutrophil) ELISA Kit

catalog :

MBS2504270

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2504270

products type :

ELISA Kit

products full name :

Human NE/ELA2 (Elastase 2, Neutrophil) ELISA Kit

products short name :

[NE/ELA2]

other names :

[neutrophil elastase preproprotein; Neutrophil elastase; neutrophil elastase; PMN elastase; bone marrow serine protease; elastase 2, neutrophil; elastase-2; granulocyte-derived elastase; human leukocyte elastase; medullasin; polymorphonuclear elastase; elastase, neutrophil expressed; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase]

products gene name :

[NE/ELA2]

other gene names :

[ELANE; ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E; ELA2; HLE]

uniprot entry name :

ELNE_HUMAN

reactivity :

Human

sequence length :

267

specificity :

This kit recognizes Human MYH9 in samples. No significant cross-reactivity or interference between HumanMYH9and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MYH9 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MYH9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYH9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYH9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MYH9. You can calculate the concentration of Human MYH9 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4503549

ncbi acc num :

NP_001963.1

ncbi gb acc num :

NM_001972.2

uniprot acc num :

P08246

ncbi mol weight :

28,518 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); C-MYB Transcription Factor Network Pathway (138073); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Systemic Lupus Erythematosus Pathway (83122); Systemic Lupus Erythematosus Pathway (534); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]

uniprot summary :

ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily. Protein type: EC 3.4.21.37; Cell cycle regulation; Motility/polarity/chemotaxis; Cell surface; Protease. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: cell surface; transcriptional repressor complex; cytoplasm; extracellular region; secretory granule. Molecular Function: peptidase activity; heparin binding; protein binding; protease binding; serine-type endopeptidase activity; cytokine binding; endopeptidase activity. Biological Process: positive regulation of immune response; extracellular matrix organization and biogenesis; positive regulation of smooth muscle cell proliferation; negative regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 biosynthetic process; response to lipopolysaccharide; negative regulation of chemotaxis; negative regulation of transcription from RNA polymerase II promoter; proteolysis; phagocytosis; cellular calcium ion homeostasis; extracellular matrix disassembly; positive regulation of MAP kinase activity; collagen catabolic process; negative regulation of inflammatory response; defense response to bacterium; protein catabolic process; response to yeast; leukocyte migration; negative regulation of chemokine biosynthetic process; response to UV; acute inflammatory response to antigenic stimulus. Disease: Cyclic Neutropenia; Neutropenia, Severe Congenital, 1, Autosomal Dominant

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590