ELISA/assay

Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit

MBS2504028

24-Strip-Wells

190 USD

ELISA Kit

Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit

[TIMP-1]

[TIMP-1; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; EG-1; TIMP-1; embryogenin-1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); tissue inhibitor of metalloproteinases 1; TIMP metallopeptidase inhibitor 1<; Embryogenin-1; EG-1; Tissue inhibitor of metalloproteinases 1; TIMP-1]

[TIMP-1]

[TIMP1; TIMP1; EG-1; TIMP-1]

TIMP1_BOVIN

Human

207

This kit recognizes natural and some recombinant Human TIMP-1. No significant cross-reactivity or interference between Human TIMP-1 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human TIMP-1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-1. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human TIMP-1 and Avidin- Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TIMP-1. The concentration of Human TIMP-1 in samples can be calculated by comparing the OD of the samples with the standard curve.

546974

AAB30892.1

P20414

23,032 Da

Activation Of Matrix Metalloproteinases Pathway (1120055); Degradation Of The Extracellular Matrix Pathway (1120054); Extracellular Matrix Organization Pathway (1120041); HIF-1 Signaling Pathway (695207); Hemostasis Pathway (1120392); Matrix Metalloproteinases Pathway (198565); Platelet Activation, Signaling And Aggregation Pathway (1120402); Platelet Degranulation Pathway (1120414); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1120412)

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.

24-Strip-Wells

190 USD

48-Strip-Wells

310

96-Strip-Wells

355

5x96-Strip-Wells

1435

10x96-Strip-Wells

2805