ELISA/assay

Porcine IL-17 (Interleukin 17) ELISA Kit

MBS2504020

48-Strip-Wells

400 USD

ELISA Kit

Porcine IL-17 (Interleukin 17) ELISA Kit

IL-17

interleukin-17A; Interleukin-17A; interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8

IL-17

IL17A; IL17A; IL17; CTLA8; IL-17; IL-17A; CTLA8; IL17; IL-17; IL-17A; CTLA-8

IL17_HUMAN

Porcine

155

This kit recognizes natural and recombinant Porcine IL-17. No significant cross-reactivity or interference between Porcine IL-17 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: The minimum detectable dose of Porcine IL-17 is 9.375pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine IL-17 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-17. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-17 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-17, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-17. You can calculate the concentration of IL-17 in the samples by comparing the OD of the samples to the standard curve.

4504651

NP_002181.1

NM_002190.2

Q16552

17,504 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL17 Signaling Pathway (920968); IL23-mediated Signaling Events Pathway (138000); IL27-mediated Signaling Events Pathway (137929); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Rheumatoid Arthritis Pathway (200309); Rheumatoid Arthritis Pathway (200269); TCR Signaling Pathway (198862)

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6p12. Cellular Component: extracellular space; external side of plasma membrane. Molecular Function: cytokine activity. Biological Process: cell death; cell-cell signaling; positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; apoptosis; protein amino acid glycosylation; immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response

48-Strip-Wells

400 USD

96-Strip-Wells

475