catalog number :
MBS2503928
products type :
ELISA Kit
products full name :
Rat ATGL (Adipose Triglyceride Lipase) ELISA Kit
products short name :
[ATGL]
other names :
[ATGL; ATGL; patatin-like phospholipase domain containing 2; ATGL]
products gene name :
[ATGL]
other gene names :
[PNPLA2; ATGL]
uniprot entry name :
B3VHV7_ANAPL
specificity :
This kit recognizes natural and some recombinant Rat ATGL. No significant cross-reactivity or interference between Rat ATGL and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat ATGL concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ATGL. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rat ATGL and Avidin- Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rat ATGL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzymesubstrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat ATGL. The concentration of Rat ATGL in samples can be calculated by comparing the OD of the samples with the standard curve.
ncbi acc num :
ACE80204.2
ncbi mol weight :
53,198 Da
ncbi pathways :
Acylglycerol Degradation Pathway (884871); Acylglycerol Degradation Pathway (468272); Glycerolipid Metabolism Pathway (871121); Glycerolipid Metabolism Pathway (361); Metabolic Pathways (871160)
size5 :
10x96-Strip-Wells