Rat NT-Pro-ANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit | MyBioSource MBS2503904 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat NT-Pro-ANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

catalog :

MBS2503904

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2503904

products type :

ELISA Kit

products full name :

Rat NT-Pro-ANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

products short name :

[NT-Pro-ANP]

other names :

[natriuretic peptides B preproprotein; Natriuretic peptides B; natriuretic peptides B; brain type natriuretic peptide; gamma-brain natriuretic peptide; natriuretic peptide precursor B; natriuretic protein; natriuretic peptide B; Gamma-brain natriuretic peptideCleaved into the following 16 chains:Brain natriuretic peptide 32; BNP(1-32); BNP-32; BNP(1-30); BNP(1-29); BNP(1-28); BNP(2-31); BNP(3-32); BNP(3-30); BNP(3-29); BNP(4-32); BNP(4-31); BNP(4-30); BNP(4-29); BNP(4-27); BNP(5-32); BNP(5-31); BNP(5-29)]

products gene name :

[NT-pro-ANP]

other gene names :

[NPPB; NPPB; BNP; BNP(1-32); BNP-32]

uniprot entry name :

ANFB_HUMAN

reactivity :

Rat

sequence length :

134

specificity :

This kit recognizes Rat NSE in samples. No significant cross-reactivity or interference between Rat NSE and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20ng/mL. Sensitivity: 0.19ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level Rat NSE were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level Rat NSE were testedon 3 different plates, 20 replicates in eachplate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat NSE concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NSE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat NSE. You can calculate the concentration of Rat NSE in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4505433

ncbi acc num :

NP_002512.1

ncbi gb acc num :

NM_002521.2

uniprot acc num :

P16860

ncbi mol weight :

14,726 Da

ncbi pathways :

MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); CGMP-PKG Signaling Pathway (983748); CGMP-PKG Signaling Pathway (985584)

ncbi summary :

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]

uniprot summary :

NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family. Protein type: Secreted; Secreted, signal peptide; Hormone. Chromosomal Location of Human Ortholog: 1p36.2. Cellular Component: extracellular space; protein complex; extracellular region. Molecular Function: diuretic hormone activity; hormone activity; receptor binding. Biological Process: cGMP biosynthetic process; negative regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of vasodilation; regulation of blood pressure; receptor guanylyl cyclase signaling pathway; regulation of vascular permeability; negative regulation of cell growth; body fluid secretion; regulation of blood vessel size

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590