ELISA/assay

Human FOXP3 (Forkhead Box Protein P3) ELISA Kit

MBS2503897

24-Strip-Wells

215 USD

ELISA Kit

Human FOXP3 (Forkhead Box Protein P3) ELISA Kit

[FOXP3]

[forkhead box protein P3 isoform a; Forkhead box protein P3; forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; scurfin; forkhead box P3; Scurfin]

[FOXP3]

[FOXP3; FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER; IPEX]

FOXP3_HUMAN

Human

431

This kit recognizes Human FGL2 in samples. No Significant cross-reactivity or interference between Human FGL2 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 46.88-3000 pg/mL. Sensitivity: 28.13 pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human FGL2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human FGL2 were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FGL2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FGL2. You can calculate the concentration of Human FGL2 in the samples by comparing the OD of the samples to the standard curve.

31982943

NP_054728.2

NM_014009.3

Q9BZS1

44,407 Da

Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); IL2 Signaling Events Mediated By STAT5 Pathway (137988); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797)

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Transcription factor; C2H2-type zinc finger protein; DNA-binding. Chromosomal Location of Human Ortholog: Xp11.23. Cellular Component: protein complex; cytoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; NF-kappaB binding; histone acetyltransferase binding; protein binding; protein homodimerization activity; NFAT protein binding; sequence-specific DNA binding; metal ion binding; histone deacetylase binding; transcription factor activity; transcription corepressor activity. Biological Process: negative regulation of interleukin-10 production; positive regulation of transcription, DNA-dependent; myeloid cell homeostasis; negative regulation of cytokine secretion; T cell receptor signaling pathway; negative regulation of interleukin-2 production; negative regulation of interleukin-6 production; T cell homeostasis; tolerance induction to self antigen; response to virus; negative regulation of transcription factor activity; negative regulation of immune response; cytokine production; negative regulation of T cell cytokine production; positive regulation of peripheral T cell tolerance induction; inhibition of NF-kappaB transcription factor; regulation of T cell anergy; negative regulation of interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; inhibition of CREB transcription factor; negative regulation of transcription, DNA-dependent; negative regulation of cytokine biosynthetic process; transcription from RNA polymerase II promoter; negative regulation of interleukin-4 production; T cell activation; negative regulation of activated T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of histone acetylation; negative regulation of interferon-gamma biosynthetic process; positive regulation of interleukin-4 production; negative regulation of cell proliferation; negative regulation of isotype switching to IgE isotypes; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; negative regulation of T cell proliferation; regulation of transcription, DNA-dependent; B cell homeostasis; negative regulation of interferon-gamma production; positive regulation of T cell anergy; regulation of isotype switching to IgG isotypes; positive regulation of transforming growth factor-beta1 production; negative regulation of chronic inflammatory response; negative regulation of histone deacetylation; T cell mediated immunity; positive regulation of immature T cell proliferation in the thymus; negative regulation of tumor necrosis factor production; chromatin remodeling; negative regulation of interleukin-17 production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; negative regulation of interleukin-2 biosynthetic process; positive regulation of histone acetylation. Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590