catalog number :
MBS2503828
products type :
ELISA Kit
products full name :
Porcine FETUB (Fetuin B) ELISA Kit
products short name :
FETUB
other names :
fetuin-B; Fetuin-B; fetuin-B; fetuin-like protein IRL685; fetuin B; 16G2; Fetuin-like protein IRL685; Gugu
products gene name :
FETUB
other gene names :
FETUB; FETUB; 16G2; Gugu; IRL685
uniprot entry name :
FETUB_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Porcine FETUB. No significant cross-reactivity or interference between Porcine FETUB and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Porcine FETUB and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313--20ng/mL. Sensitivity: 0.188ng/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FETUB in Porcine serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine FETUB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Porcine FETUB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine FETUB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine FETUB. You can calculate the concentration of Porcine FETUB in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_055190.2
ncbi gb acc num :
NM_014375.2
ncbi mol weight :
37,823 Da
ncbi summary :
The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells. [provided by RefSeq, Jul 2008]
uniprot summary :
FETUB: is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells. [provided by RefSeq, Jul 2008]. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3q27. Cellular Component: extracellular space. Molecular Function: metalloendopeptidase inhibitor activity; cysteine protease inhibitor activity. Biological Process: binding of sperm to zona pellucida; single fertilization