catalog number :
MBS2503794
products type :
ELISA Kit
products full name :
Rat BNP (Brain Natriuretic Peptide) ELISA Kit
products short name :
[BNP]
other names :
[brain natriuretic peptide; Brain natriuretic peptide; brain natriuretic peptide; B-type natriuretic peptide]
products gene name :
[BNP]
other gene names :
[nppb; nppb; bnp; bnp]
uniprot entry name :
ANFB_TAKRU
specificity :
This kit recognizes Mouse PINP in samples. No significant cross-reactivity or interference between Mouse PINP and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 93.75-6000pg/mL. Sensitivity: 56.25pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level MousePINP were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level MousePINP were testedon 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse PINP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PINP. St andards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PINP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PINP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PINP. You can calculate the concentration of Mouse PINP in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001027860.1
ncbi gb acc num :
NM_001032688.1
ncbi mol weight :
14,746 Da
uniprot summary :
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Has a cGMP-stimulating activity ().
size5 :
10x96-Strip-Wells