Human SFRP2 (Secreted Frizzled Related Protein 2) ELISA Kit | MyBioSource MBS2503785 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human SFRP2 (Secreted Frizzled Related Protein 2) ELISA Kit

catalog :

MBS2503785

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2503785

products type :

ELISA Kit

products full name :

Human SFRP2 (Secreted Frizzled Related Protein 2) ELISA Kit

products short name :

SFRP2

other names :

secreted frizzled-related protein 2; Secreted frizzled-related protein 2; secreted frizzled-related protein 2; SARP-1; sFRP-2; secreted apoptosis related protein 1; secreted apoptosis-related protein 1; secreted frizzled-related protein 2; Secreted apoptosis-related protein 1; SARP-1

products gene name :

SFRP2

other gene names :

SFRP2; SFRP2; FRP-2; SARP1; SDF-5; FRP2; SARP1; FRP-2; sFRP-2; SARP-1

uniprot entry name :

SFRP2_HUMAN

reactivity :

Human

sequence length :

295

specificity :

This kit recognizes natural and recombinant Human SFRP2. No significant cross-reactivity or interference between Human SFRP2 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Detection Range: 1.25-80ng/mL. Sensitivity: The minimum detectable dose of Human SFRP2 is 0.75ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). Assay Type: Sandwich

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SFRP2 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SFRP2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SFRP2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SFRP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SFRP2. You can calculate the concentration of SFRP2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

48475052

ncbi acc num :

NP_003004.1

ncbi gb acc num :

NM_003013.2

uniprot acc num :

Q96HF1

ncbi mol weight :

33,490 Da

ncbi pathways :

Disease Pathway (530764); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1127632); Signal Transduction Pathway (477114); Signaling By WNT In Cancer Pathway (1127614); Signaling By Wnt Pathway (106510); TCF Dependent Signaling In Response To WNT Pathway (1127516); Wnt Signaling Pathway NetPath (198799); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471); XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway (1127633)

ncbi summary :

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq, Jul 2008]

uniprot summary :

SFRP2: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. Belongs to the secreted frizzled-related protein (sFRP) family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q31.3. Cellular Component: extracellular matrix; extracellular space; integral to membrane. Molecular Function: G-protein coupled receptor activity; integrin binding; Wnt-protein binding; Wnt receptor activity; fibronectin binding; receptor agonist activity. Biological Process: convergent extension involved in axis elongation; collagen fibril organization; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; negative regulation of caspase activity; negative regulation of BMP signaling pathway; negative regulation of cell proliferation; cell-cell signaling; negative regulation of mesodermal cell fate specification; positive regulation of cell proliferation; negative regulation of cell migration; response to nutrient; negative regulation of epithelial cell proliferation; response to drug; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of JNK activity; male gonad development; positive regulation of cell adhesion mediated by integrin; digestive tract morphogenesis; chondrocyte development; positive regulation of peptidyl-serine phosphorylation; positive regulation of cell growth; patterning of blood vessels; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; neural tube closure; cellular response to extracellular stimulus; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage