ELISA/assay

Rabbit CASP1 (Caspase 1) ELISA Kit

MBS2503782

48-Strip-Wells

450 USD

ELISA Kit

Rabbit CASP1 (Caspase 1) ELISA Kit

CASP1

caspase-1 isoform beta; Caspase-1; caspase-1; CASP1 nirs variant 1; IL-1 beta-converting enzyme; IL1B-convertase; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); interleukin 1-B converting enzyme; caspase 1, apoptosis-related cysteine peptidase; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45Cleaved into the following 2 chains:Caspase-1 subunit p20; Caspase-1 subunit p10

CASP1

CASP1; CASP1; ICE; P45; IL1BC; IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme

CASP1_HUMAN

Rabbit

383

This assay has high sensitivity and excellent specificity for detection of Rabbit CASP1. No significant cross-reactivity or interference between Rabbit CASP1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rabbit CASP1 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 62.5--4000pg/mL. Sensitivity: 37.5pg/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP1 in Rabbit serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit CASP1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rabbit CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit CASP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rabbit CASP1. You can calculate the concentration of Rabbit CASP1 in the samples by comparing the OD of the samples to the standard curve.

380254459

NP_001244048.1

NM_001257119.2

P29466

10,417 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Caspase Cascade In Apoptosis Pathway (137974); Cellular Roles Of Anthrax Toxin Pathway (138076); Cytokine Signaling In Immune System Pathway (366171); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Direct P53 Effectors Pathway (137939)

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.22.36; Apoptosis; Protease. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: cytoplasm; extracellular region; cytosol. Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; caspase activator activity. Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; membrane hyperpolarization; interleukin-1 beta production; response to lipopolysaccharide; mitochondrial depolarization; positive regulation of interleukin-1 beta secretion; signal transduction; proteolysis; response to ATP; positive regulation of interleukin-1 alpha secretion; regulation of inflammatory response; response to hypoxia; innate immune response; regulation of autophagy

48-Strip-Wells

450 USD

96-Strip-Wells

535