ELISA/assay

Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit

MBS2503629

48-Strip-Wells

410 USD

ELISA Kit

Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit

IGFBP-5

insulin-like growth factor-binding protein 5; Insulin-like growth factor-binding protein 5; insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5; insulin-like growth factor binding protein 5

IGFBP-5

IGFBP5; IGFBP5; IBP5; IBP5; IBP-5; IGF-binding protein 5; IGFBP-5

IBP5_HUMAN

Human

272

This assay has high sensitivity and excellent specificity for detection of Human IGFBP-5. No significant cross-reactivity or interference between Human IGFBP-5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human IGFBP-5 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563--100ng/mL. Sensitivity: 0.938ng/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IGFBP-5 in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IGFBP-5. You can calculate the concentration of Human IGFBP-5 in the samples by comparing the OD of the samples to the standard curve.

10834982

NP_000590.1

NM_000599.3

P24593

30,570 Da

Metabolism Of Proteins Pathway (106230); Myometrial Relaxation And Contraction Pathways (198759); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908); Senescence And Autophagy Pathway (198780)

IGFBP5: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted. Chromosomal Location of Human Ortholog: 2q35. Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region. Molecular Function: protein binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding. Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; mammary gland involution; hair follicle morphogenesis; negative regulation of smooth muscle cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; glucose metabolic process; negative regulation of smooth muscle cell migration; glucose homeostasis; signal transduction; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; negative regulation of translation; striated muscle cell differentiation; negative regulation of osteoblast differentiation; regulation of cell growth; skeletal muscle growth; negative regulation of cell migration

48-Strip-Wells

410 USD

96-Strip-Wells

490