ELISA/assay

Porcine MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

MBS2503610

24-Strip-Wells

235 USD

ELISA Kit

Porcine MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

[MCP-1]

[MCP-1; C-C motif chemokine 2; C-C motif chemokine 2; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte chemotactic protein 1; monocyte secretory protein JE; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small-inducible cytokine A2; chemokine (C-C motif) ligand 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2]

[MCP-1]

[CCL2; CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30; MCP1; SCYA2; MCAF; MCP-1]

CCL2_HUMAN

Porcine

99

This kit recognizes Porcine KIM-1 in samples. No significant cross-reactivity or interference between Porcine KIM-1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Porcine KIM-1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Porcine KIM-1 were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine KIM-1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine KIM-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine KIM-1. You can calculate the concentration of Porcine KIM-1 in the samples by comparing the OD of the samples to the standard curve.

545465

AAB29926.1

P13500

11,025 Da

ATF4 Activates Genes Pathway (530772); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664)

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]

CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine; Secreted. Chromosomal Location of Human Ortholog: 17q11.2-q12. Cellular Component: extracellular space; rough endoplasmic reticulum; endocytic vesicle; perinuclear region of cytoplasm; dendrite; extracellular region; synapse; perikaryon; nerve terminal. Molecular Function: heparin binding; chemokine activity; CCR2 chemokine receptor binding; receptor binding; protein kinase activity. Biological Process: maternal process involved in pregnancy; protein amino acid phosphorylation; response to antibiotic; regulation of cell shape; monocyte chemotaxis; cell surface receptor linked signal transduction; response to vitamin B3; transforming growth factor beta receptor signaling pathway; negative regulation of neuron apoptosis; cellular homeostasis; cell adhesion; neutrophil chemotaxis; organ regeneration; JAK-STAT cascade; response to amino acid stimulus; positive regulation of tumor necrosis factor production; G-protein signaling, coupled to cyclic nucleotide second messenger; organ morphogenesis; unfolded protein response, activation of signaling protein activity; response to ethanol; response to bacterium; cellular response to insulin stimulus; response to mechanical stimulus; response to heat; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; chemotaxis; signal transduction; positive regulation of synaptic transmission; positive regulation of cellular extravasation; response to wounding; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; response to gamma radiation; angiogenesis; inflammatory response; lymphocyte chemotaxis; aging; unfolded protein response; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; viral genome replication; macrophage chemotaxis; leukocyte migration during inflammatory response; humoral immune response; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of leukocyte mediated cytotoxicity; negative regulation of angiogenesis; cellular protein metabolic process; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration. Disease: Neural Tube Defects; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To

24-Strip-Wells

235 USD

48-Strip-Wells

450

96-Strip-Wells

535

5x96-Strip-Wells

2255

10x96-Strip-Wells

3975