catalog number :
MBS2503609
products type :
ELISA Kit
products full name :
Human FPA (Fibrinopeptide A) ELISA Kit
products short name :
FPA
other names :
Flowering time control protein FPA; Flowering time control protein FPA; Flowering time control protein FPA
other gene names :
FPA; FPA; T1O24.15
uniprot entry name :
FPA_ARATH
specificity :
This kit recognizes natural and recombinant Human FPA. No significant cross-reactivity or interference between Human FPA and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/mL. Sensitivity: The minimum detectable dose of Human FPA is 0.938ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FPA concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FPA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FPA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FPA. You can calculate the concentration of FPA in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_181869.2
ncbi gb acc num :
NM_129902.2
ncbi mol weight :
62,451 Da
ncbi pathways :
Flowering Time Pathway (760638)
ncbi summary :
FPA is a gene that regulates flowering time in Arabidopsis via a pathway that is independent of daylength (the autonomous pathway). Mutations in FPA result in extremely delayed flowering. Double mutants with FCA have reduced fertility and single/double mutants have defects in siRNA mediated chromatin silencing.