catalog number :
MBS2503607
products type :
ELISA Kit
products full name :
Human OCT4 (Octamer Binding Transcription Factor 4) ELISA Kit
products short name :
4-Oct
other names :
OCT4 protein; OCT4 protein
products gene name :
4-Oct
specificity :
This kit recognizes natural and recombinant Human OCT4. No significant cross-reactivity or interference between Human OCT4 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Human OCT4 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human OCT4 concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OCT4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OCT4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OCT4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of OCT4. You can calculate the concentration of OCT4 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001272498.1
ncbi gb acc num :
NM_001285569.1
ncbi pathways :
Signaling Pathways Regulating Pluripotency Of Stem Cells (1026269); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502)