catalog number :
MBS2503504
products type :
ELISA Kit
products full name :
Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit
products short name :
FABP1
other names :
fatty acid-binding protein, liver; Fatty acid-binding protein, liver; fatty acid-binding protein, liver; fatty acid-binding protein 1; liver-type fatty acid-binding protein; fatty acid binding protein 1, liver; Fatty acid-binding protein 1; Liver-type fatty acid-binding protein; L-FABP
products gene name :
FABP1
other gene names :
FABP1; FABP1; FABPL; L-FABP; FABPL; L-FABP
uniprot entry name :
FABPL_HUMAN
specificity :
This kit recognizes natural and recombinant Human FABP1. No significant cross-reactivity or interference between Human FABP1 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 6.25-400ng/mL. Sensitivity: 3.75ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FABP1 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FABP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FABP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FABP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FABP1. You can calculate the concentration of FABP1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001434.1
ncbi gb acc num :
NM_001443.2
ncbi mol weight :
14,208 Da
ncbi pathways :
Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450); PPARA Activates Gene Expression Pathway (530749); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544); Selenium Metabolism And Selenoproteins Pathway (198846)
ncbi summary :
This gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Mar 2011]
uniprot summary :
FABP1: Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. Protein type: Lipid-binding. Chromosomal Location of Human Ortholog: 2p11. Cellular Component: nucleoplasm; peroxisomal matrix; apical cortex; cytosol. Molecular Function: antioxidant activity; bile acid binding; phospholipid binding; long-chain fatty acid transporter activity; chromatin binding; drug binding; fatty acid binding. Biological Process: long-chain fatty acid transport; positive regulation of cell proliferation; positive regulation of hydrolase activity; negative regulation of caspase activity; cellular lipid metabolic process; intestinal absorption; positive regulation of fatty acid beta-oxidation; negative regulation of apoptosis