Mouse PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit | MyBioSource MBS2503456 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit

catalog :

MBS2503456

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2503456

products type :

ELISA Kit

products full name :

Mouse PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit

products short name :

PACAP

other names :

PACAP; Pituitary adenylate cyclase-activating polypeptide; pituitary adenylate cyclase-activating polypeptide; prepro-PACAP; adenylate cyclase activating polypeptide 1 (pituitary); PRP-48

products gene name :

PACAP

other gene names :

ADCYAP1; ADCYAP1; PACAP; PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38

uniprot entry name :

PACA_HUMAN

reactivity :

Mouse

sequence length :

176

specificity :

This kit recognizes natural and recombinant Mouse PACAP. No significant cross-reactivity or interference between Mouse PACAP and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: The minimum detectable dose of Mouse PACAP is 9.375pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PACAP concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PACAP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PACAP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PACAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PACAP. You can calculate the concentration of PACAP in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

1132550

ncbi acc num :

CAA42962.1

uniprot acc num :

P18509

ncbi mol weight :

18,835 Da

ncbi pathways :

Activation Of TRKA Receptors Pathway (106456); Class B/2 (Secretin Family Receptors) Pathway (106378); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Insulin Secretion Pathway (777534); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637)

ncbi summary :

This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]

uniprot summary :

ADCYAP1: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Belongs to the glucagon family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 18p11. Cellular Component: extracellular space; extracellular region; terminal button. Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor signaling protein activity; pituitary adenylate cyclase-activating peptide receptor binding; pituitary adenylate cyclase activating polypeptide activity; receptor binding. Biological Process: behavioral fear response; nerve growth factor receptor signaling pathway; negative regulation of potassium ion transport; female pregnancy; sensory perception of pain; positive regulation of vasodilation; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell cycle; histamine secretion; cAMP-mediated signaling; elevation of cytosolic calcium ion concentration; regulation of protein localization; cell-cell signaling; ovarian follicle development; neuropeptide signaling pathway; positive regulation of cell proliferation; neurite development; adenylate cyclase activation; negative regulation of acute inflammatory response to antigenic stimulus; positive regulation of interleukin-6 production; positive regulation of growth hormone secretion; ATP metabolic process; response to starvation; response to ethanol; regulation of postsynaptic membrane potential; pituitary gland development; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of acute inflammatory response to non-antigenic stimulus; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of G-protein coupled receptor protein signaling pathway

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage