Mouse AIF (Apoptosis Inducing Factor) ELISA Kit | MyBioSource MBS2503405 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse AIF (Apoptosis Inducing Factor) ELISA Kit

catalog :

MBS2503405

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2503405

products type :

ELISA Kit

products full name :

Mouse AIF (Apoptosis Inducing Factor) ELISA Kit

products short name :

[AIF]

other names :

[apoptosis-inducing factor 1, mitochondrial isoform 1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor 1, mitochondrial; Neuropathy, axonal motor-sensory, with deafness and mental retardation; neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome); programmed cell death 8 (apoptosis-inducing factor); striatal apoptosis-inducing factor; apoptosis-inducing factor, mitochondrion-associated, 1; Programmed cell death protein 8]

products gene name :

[AIF]

other gene names :

[AIFM1; AIFM1; AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6; AIF; PDCD8]

uniprot entry name :

AIFM1_HUMAN

reactivity :

Mouse

sequence length :

613

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse AIF. No significant cross-reactivity or interference between Mouse AIF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse AIF and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 7.813-500pg/mL. Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AIF in Mouse serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AIF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse AIF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse AIF. You can calculate the concentration of Mouse AIF in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4757732

ncbi acc num :

NP_004199.1

ncbi gb acc num :

NM_004208.3

uniprot acc num :

O95831

ncbi mol weight :

26,033 Da

ncbi pathways :

Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Modulation By HSP70 Pathway (198877); Ceramide Signaling Pathway (138023)

ncbi summary :

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]

uniprot summary :

PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces parthanatos i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial; Apoptosis; EC 1.-.-.-; Oxidoreductase. Chromosomal Location of Human Ortholog: Xq26.1. Cellular Component: mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space; nucleus; cytosol. Molecular Function: protein dimerization activity; protein binding; oxidoreductase activity, acting on NADH or NADPH; electron carrier activity; DNA binding; NAD(P)H oxidase activity. Biological Process: chromosome condensation; caspase activation; neuron differentiation; mitochondrial respiratory chain complex I assembly; neuron apoptosis; cell redox homeostasis; apoptosis; positive regulation of apoptosis; DNA fragmentation during apoptosis; DNA catabolic process. Disease: Cowchock Syndrome

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590