catalog number :
MBS2503349
products type :
ELISA Kit
products full name :
Rat IL-5 (Interleukin 5) ELISA Kit
products short name :
[IL-5]
other names :
[interleukin-5; Interleukin-5; interleukin-5; B-cell growth factor II; BCGF-II; IL-5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); T-cell replacing factor; TRF; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor; interleukin 5; B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF]
products gene name :
[IL-5]
other gene names :
[Il5; Il5; Il-5; IL-5; BCGF-II; TRF]
uniprot entry name :
IL5_RAT
specificity :
This kit recognizes natural and recombinant Rat IL-5. No significant cross-reactivity or interference between Rat IL-5 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IL-5 concentrations in serum, plasma and other biological fluids.
products description :
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-5. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-5. You can calculate the concentration of IL-5 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_068606.1
ncbi gb acc num :
NM_021834.1
ncbi mol weight :
15,207 Da
ncbi pathways :
Allograft Rejection Pathway (83511); Allograft Rejection Pathway (535); Asthma Pathway (83508); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83509); Autoimmune Thyroid Disease Pathway (533); Cytokine Signaling In Immune System Pathway (1121619); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198521)
ncbi summary :
cytokine with B-cell growth factor activity [RGD, Feb 2006]
uniprot summary :
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
size5 :
10x96-Strip-Wells