Human MYH9 (Myosin Heavy Chain 9, Non Muscle) ELISA Kit | MyBioSource MBS2503294 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human MYH9 (Myosin Heavy Chain 9, Non Muscle) ELISA Kit

catalog :

MBS2503294

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2503294

products type :

ELISA Kit

products full name :

Human MYH9 (Myosin Heavy Chain 9, Non Muscle) ELISA Kit

products short name :

[MYH9]

other names :

[myosin-9; Myosin-9; myosin-9; cellular myosin heavy chain, type A; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A; myosin, heavy chain 9, non-muscle; Cellular myosin heavy chain, type A; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Non-muscle myosin heavy chain A; NMMHC-A; Non-muscle myosin heavy chain IIa; NMMHC II-a; NMMHC-IIA]

products gene name :

[MYH9]

other gene names :

[MYH9; MYH9; MHA; FTNS; EPSTS; BDPLT6; DFNA17; NMMHCA; NMHC-II-A; NMMHC-IIA; NMMHC-A; NMMHC II-a; NMMHC-IIA]

uniprot entry name :

MYH9_HUMAN

reactivity :

Human

sequence length :

1960

specificity :

This kit recognizes Human MVP in samples. No Significant cross-reactivity or interference between Human MVP and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10 ng/mL. Sensitivity: 0.1 ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MVP were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MVP were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MVP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MVP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MVP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MVP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MVP. You can calculate the concentration of Human MVP in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

12667788

ncbi acc num :

NP_002464.1

ncbi gb acc num :

NM_002473.5

uniprot acc num :

P35579

ncbi mol weight :

159,864 Da

ncbi pathways :

Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1127691); EPHA-mediated Growth Cone Collapse Pathway (1127692); Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway (771577); Immune System Pathway (106386); Innate Immune System Pathway (106387); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway (771579)

ncbi summary :

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]

uniprot summary :

MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: signalosome; cortical cytoskeleton; protein complex; myosin II complex; leading edge; contractile ring; immunological synapse; actomyosin; cytosol; actin cytoskeleton; ruffle; cell-cell adherens junction; membrane; cytoplasm; plasma membrane; stress fiber; spindle; uropod; neuromuscular junction; integrin complex; nucleus; cleavage furrow. Molecular Function: microfilament motor activity; calmodulin binding; actin filament binding; protein binding; protein homodimerization activity; protein anchor; ATPase activity; motor activity; actin binding; ADP binding; actin-dependent ATPase activity; ATP binding. Biological Process: integrin-mediated signaling pathway; axon guidance; monocyte differentiation; blood vessel endothelial cell migration; in utero embryonic development; actin filament-based movement; actomyosin structure organization and biogenesis; membrane protein ectodomain proteolysis; cytokinesis; uropod organization and biogenesis; establishment of meiotic spindle localization; regulation of cell shape; protein transport; actin cytoskeleton reorganization; ephrin receptor signaling pathway; establishment of T cell polarity; angiogenesis; platelet formation; leukocyte migration; myoblast fusion; meiotic spindle organization and biogenesis. Disease: Deafness, Autosomal Dominant 17; May-hegglin Anomaly; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; Epstein Syndrome; Sebastian Syndrome

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590