catalog number :
MBS2503246
products type :
ELISA Kit
products full name :
Human IgA1 (Immunoglobulin A1) ELISA Kit
products short name :
IgA1
other names :
immunoglobin A1 protease; Immunoglobulin A1 protease autotransporter; immunoglobin A1 protease; Helper peptide
products gene name :
IgA1
other gene names :
iga1; iga; iga1; IGA1 protease
uniprot entry name :
IGA0_HAEIN
specificity :
This kit recognizes natural and recombinant Human IgA1. No significant cross-reactivity or interference between Human IgA1 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 46.875-3000ng/mL. Sensitivity: The minimum detectable dose of Human IgA1 is 28.125ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IgA1 concentrations in serum, plasma and other biological fluids.
products description :
. Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IgA1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IgA1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IgA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IgA1. You can calculate the concentration of IgA1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_439153.1
ncbi gb acc num :
NC_000907.1
ncbi mol weight :
185,539 Da