ELISA/assay

Rat GLUT1 (Glucose Transporter 1) ELISA Kit

MBS2503235

24-Strip-Wells

215 USD

ELISA Kit

Rat GLUT1 (Glucose Transporter 1) ELISA Kit

[GLUT1]

[glucose transporter-like protein I, partial; Solute carrier family 2, facilitated glucose transporter member 1; solute carrier family 2, facilitated glucose transporter member 1; glucose transporter type 1, erythrocyte/brain; hepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter]

[Glut1]

[SLC2A1; SLC2A1; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; GLUT1DS; GLUT1; GLUT-1]

GTR1_HUMAN

Rat

130

This kit recognizes natural and recombinant Rat GLUT1. No significant cross-reactivity or interference between Rat GLUT1 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.25-80ng/mL. Sensitivity: Min: 0.75ng/mL; Max: 80ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GLUT1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GLUT1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GLUT1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GLUT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GLUT1. You can calculate the concentration of GLUT1 in the samples by comparing the OD of the samples to the standard curve.

14268550

AAK56795.1

P11166

54,084 Da

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001)

This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]

GLUT1: an integral membrane protein that plays an important role in the glycolytic pathway by serving as a uniporter for glucose. One of 13 members of the human equilibrative glucose transport protein family. Transports a wide range of aldoses, including both pentoses and hexoses, and dehydroascorbic acid. Shown to transport water against an osmotic gradient. A receptor for the Human T-cell Leukemia virus (HTLV). Plays a role in the constitutive or basal uptake of glucose. Expressed at highest levels in proliferating cells of the early developing embryo, cells forming the blood tissue barriers, in human erythrocytes, astrocytes and in cardiac muscle. GLUT1 and GLUT3 are both essential for normal embryonic development. Is practically the only member of the GLUT family expressed on human red blood cells, where it comprises 10 - 20% of the integral membrane protein content. Several glycolytic proteins including the transporters GLUT1 and GLUT3, as well as multiple enzymes including HK2, PFKL, LDHA, ALDOA, ALDOC, PGK1, ENO1, PKM2, CA9 and PFKFB3 are induced in cancer cells by HIF-1 alpha. Polyps from Peutz-Jeghers patients exhibit up-regulated mTORC1 signaling, HIF-1alpha, and GLUT1 levels. Defects in GLUT1 are the cause of autosomal dominant GLUT1 deficiency syndrome, a blood-brain barrier glucose transport defect characterized by infantile seizures, delayed development, and acquired microcephaly. Defects also cause dystonia type 18, an exercise-induced paroxysmal dystonia/dyskinesia. Cytochalasin B binds to its inner surface, inhibiting its glucose transport activity with an IC50 of 0.44 uM. Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family. Chromosomal Location of Human Ortholog: 1p34.2. Cellular Component: cortical actin cytoskeleton; membrane; basolateral plasma membrane; integral to plasma membrane; melanosome; plasma membrane; female pronucleus; midbody; intercellular junction; caveola; cytosol. Molecular Function: D-glucose transmembrane transporter activity; identical protein binding; xenobiotic transporter activity; protein binding; protein self-association; dehydroascorbic acid transporter activity; glucose transmembrane transporter activity; kinase binding. Biological Process: vitamin metabolic process; L-ascorbic acid metabolic process; pathogenesis; glucose transport; response to osmotic stress; cellular response to glucose starvation; hexose transport; carbohydrate metabolic process; energy reserve metabolic process; protein complex assembly; xenobiotic transport; regulation of insulin secretion; transmembrane transport; water-soluble vitamin metabolic process. Disease: Epilepsy, Idiopathic Generalized, Susceptibility To, 12; Dystonia 9; Glut1 Deficiency Syndrome 1; Glut1 Deficiency Syndrome 2

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590