catalog number :
MBS2503170
products type :
ELISA Kit
products full name :
Porcine ACE (Angiotensin I Converting Enzyme) ELISA Kit
products short name :
[ACE]
other names :
[Ace; Accessory cholera enterotoxin]
products gene name :
[ACE]
other gene names :
[ace; Ace]
uniprot entry name :
ACE_VIBCH
specificity :
This kit recognizes natural and recombinantPorcineACE. No significant cross-reactivity or interference between PorcineACE and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of PorcineACEconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ACE. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ACEand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ACE.You can calculate the concentration of ACEin the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
CAA80292.1
ncbi mol weight :
11,347 Da
uniprot summary :
Increases short-circuit current in rabbit ileal tissue mounted in Ussing chambers, by increasing the potential difference. Cultures of V.cholerae containing the cloned ace gene cause fluid secretion in ligated rabbit ileal loops.
size5 :
10x96-Strip-Wells