catalog number :
MBS2503157
products type :
ELISA Kit
products full name :
Rat LTF/LF (Lactoferrin) ELISA Kit
products short name :
[LTF/LF]
other names :
[lactotransferrin isoform 1; Lactotransferrin; lactotransferrin; epididymis luminal protein 110; growth-inhibiting protein 12; kaliocin-1; lactoferricin; lactoferroxin; neutrophil lactoferrin; talalactoferrin; lactotransferrin; Growth-inhibiting protein 12; TalalactoferrinCleaved into the following 5 chains:Lactoferricin-H; Lfcin-H; Kaliocin-1; Lactoferroxin-A; Lactoferroxin-B; Lactoferroxin-C]
products gene name :
[LF/LTF]
other gene names :
[LTF; LTF; LF; HLF2; GIG12; HEL110; GIG12; LF; Lactoferrin; Lfcin-H]
uniprot entry name :
TRFL_HUMAN
specificity :
This kit recognizes Rat LDL-C in samples. No significant cross-reactivity or interference between Rat LDL-C and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000ng/mL. Sensitivity: 18.75ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat LDL-C were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat LDL-C were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat LDL-C concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LDL-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LDL-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LDL-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat LDL-C. You can calculate the concentration of Rat LDL-C in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_002334.2
ncbi gb acc num :
NM_002343.4
ncbi mol weight :
73,161 Da
ncbi pathways :
Amyloids Pathway (366238); Disease Pathway (530764); Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway (645267); Mtb Iron Assimilation By Chelation Pathway (1127564); Phagosomal Maturation (early Endosomal Stage) Pathway (645268); Response Of Mtb To Phagocytosis Pathway (1127560)
ncbi summary :
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
uniprot summary :
LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter. Protein type: Protease; Secreted, signal peptide; EC 3.4.21.-; Secreted. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule. Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; ferric iron binding; DNA binding; serine-type endopeptidase activity; iron ion binding. Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; interaction with host; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; activation of NF-kappaB transcription factor; regulation of cytokine production; retinal homeostasis; positive regulation of osteoblast differentiation; negative regulation of viral genome replication; innate immune response in mucosa; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; negative regulation of viral reproduction; iron assimilation by chelation and transport; negative regulation of ATPase activity; negative regulation of apoptosis
size5 :
10x96-Strip-Wells