ELISA/assay

Human S100B (S100 Calcium Binding Protein B) ELISA Kit

MBS2503148

96-Strip-Wells

445 USD

ELISA Kit

Human S100B (S100 Calcium Binding Protein B) ELISA Kit

S100B

S100B; Protein S100-B; protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein subunit beta; S100 calcium-binding protein, beta (neural); S100 calcium binding protein B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B

S100B

S100B; S100B; NEF; S100; S100-B; S100beta

S100B_HUMAN

Human

92

This assay has high sensitivity and excellent specificity for detection of Human S100B. No significant cross-reactivity or interference between Human S100B and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human S100B and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25--2000pg/mL. Sensitivity: 18.75pg/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100B in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100B. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human S100B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human S100B. You can calculate the concentration of Human S100B in the samples by comparing the OD of the samples to the standard curve.

49457242

CAG46920.1

P04271

10,713 Da

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: ruffle; extracellular space; intracellular membrane-bound organelle; cell soma; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus. Molecular Function: identical protein binding; RAGE receptor binding; protein binding; protein homodimerization activity; zinc ion binding; calcium ion binding; tau protein binding; calcium-dependent protein binding. Biological Process: regulation of cell shape; cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; learning and/or memory; central nervous system development; astrocyte differentiation; axonogenesis; positive regulation of apoptosis; positive regulation of cell proliferation; response to methylmercury; response to glucocorticoid stimulus; innate immune response; memory; regulation of neuronal synaptic plasticity

96-Strip-Wells

445 USD