Human CNR1 (Cannabinoid Receptor 1, Brain) ELISA Kit | MyBioSource MBS2503052 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human CNR1 (Cannabinoid Receptor 1, Brain) ELISA Kit

catalog :

MBS2503052

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2503052

products type :

ELISA Kit

products full name :

Human CNR1 (Cannabinoid Receptor 1, Brain) ELISA Kit

products short name :

CNR1

other names :

cannabinoid receptor 1 isoform a; Cannabinoid receptor 1; cannabinoid receptor 1; central cannabinoid receptor; cannabinoid receptor 1 (brain); CANN6

products gene name :

CNR1

other gene names :

CNR1; CNR1; CB1; CNR; CB-R; CB1A; CB1R; CANN6; CB1K5; CNR; CB-R; CB1

uniprot entry name :

CNR1_HUMAN

reactivity :

Human

sequence length :

472

specificity :

This kit recognizes natural and recombinant Human CNR1. No significant cross-reactivity or interference between Human CNR1 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.094ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CNR1 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CNR1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CNR1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CNR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CNR1. You can calculate the concentration of CNR1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

237681175

ncbi acc num :

NP_001153731.1

ncbi gb acc num :

NM_001160259.1

uniprot acc num :

P21554

ncbi mol weight :

49,060 Da

ncbi pathways :

BDNF Signaling Pathway (712093); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); N-cadherin Signaling Events Pathway (137919)

ncbi summary :

This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009]

uniprot summary :

CNR1: Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 6q14-q15. Cellular Component: growth cone; intracellular membrane-bound organelle; integral to plasma membrane; axon; plasma membrane. Molecular Function: cannabinoid receptor activity; drug binding. Biological Process: response to nicotine; negative regulation of fatty acid beta-oxidation; maternal process involved in pregnancy; regulation of synaptic transmission, glutamatergic; positive regulation of apoptosis; positive regulation of acute inflammatory response to antigenic stimulus; response to morphine; response to lipopolysaccharide; glucose homeostasis; sensory perception of pain; negative regulation of blood pressure; negative regulation of dopamine secretion; response to nutrient; aging; negative regulation of mast cell activation; negative regulation of nitric-oxide synthase activity; axonal fasciculation; negative regulation of action potential; response to cocaine; regulation of synaptic transmission, GABAergic; memory; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to cyclic nucleotide second messenger; response to ethanol; positive regulation of fever; spermatogenesis; regulation of insulin secretion; positive regulation of blood pressure

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage