catalog number :
MBS2503027
products type :
ELISA Kit
products full name :
Rat CD8 (Cluster of Differentiation 8) ELISA Kit
products short name :
[CD8]
other names :
[CD8 antigen alpha polypeptide; CD8 antigen alpha polypeptide; CD8 alpha chain; CD8 antigen, alpha polypeptide (p32); T-cell surface molecule]
products gene name :
[CD8]
other gene names :
[CD8A; CD8]
specificity :
This kit recognizes Rat CD4 in samples. No significant cross-reactivity or interference between Rat CD4 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CD4 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CD4 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat CD4 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CD4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CD4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CD4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat CD4. You can calculate the concentration of Rat CD4 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_990566.1
ncbi gb acc num :
NM_205235.1
ncbi pathways :
Cell Adhesion Molecules (CAMs) Pathway (119314); Cell Adhesion Molecules (CAMs) Pathway (480)
size5 :
10x96-Strip-Wells