ELISA/assay

Human TGM3 (Transglutaminase 3, Epidermal) ELISA Kit

MBS2503000

24-Strip-Wells

215 USD

ELISA Kit

Human TGM3 (Transglutaminase 3, Epidermal) ELISA Kit

[TGM3]

[TGM3, partial]

[TGM3]

Human

693

This kit recognizes Human TGF-alpha in samples. No significant cross-reactivity or interference between Human TGF-alpha and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TGF-alpha were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TGF-alpha were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TGF-alpha concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human TGF-alpha. You can calculate the concentration of Human TGF-alpha in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Transforming growth factor alpha (TGF-alpha) is a protein that in humans is encoded by the TGFA gene. As a member of the epidermal growth factor (EGF) family, TGF-alpha is a mitogenic polypeptide [1]. The protein becomes activated when binding to receptors capable of protein kinase activity for cellular signaling. TGF-alpha is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. TGF-alpha may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate [2]. TGF-alpha is a member of EGF family that activates the EGFR Trans membrane tyrosine kinas and leads to increase of intracellular calcium levels, glycolysis and expression of some genes such as EGFR gene which ultimately leads to DNA synthesis and cell proliferation. EGF family is involved in growth, differentiation, maintain and repair various tissues such as nervous system and gastrointestinal tract. In comparison with other ligands of this family, TGF-alpha has broader abundance and distribution in CNS[3].

649121569

AIC55234.1

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590