ELISA/assay

Rat TIMP-1(Tissue Inhibitors of Metalloproteinase 1)ELISA Kit

MBS2502910

96-Strip-Wells

445 USD

ELISA Kit

Rat TIMP-1(Tissue Inhibitors of Metalloproteinase 1)ELISA Kit

TIMP-1

TIMP-1; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; EG-1; TIMP-1; embryogenin-1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); tissue inhibitor of metalloproteinases 1; TIMP metallopeptidase inhibitor 1<; Embryogenin-1; EG-1; Tissue inhibitor of metalloproteinases 1; TIMP-1

TIMP-1

TIMP1; TIMP1; EG-1; TIMP-1

TIMP1_BOVIN

Rat

207

This kit recognizes natural and recombinant Rat TIMP-1. No significant cross-reactivity or interference between Rat TIMP-1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Rat TIMP-1 is 0.094ng/mL

Intended Uses This ELISA kit applies to the in vitro quantitative determination of Rat TIMP-1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TIMP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TIMP-1. You can calculate the concentration of TIMP-1 in the samples by comparing the OD of the samples to the standard curve.

546974

AAB30892.1

P20414

23,032 Da

Activation Of Matrix Metalloproteinases Pathway (1120055); Degradation Of The Extracellular Matrix Pathway (1120054); Extracellular Matrix Organization Pathway (1120041); HIF-1 Signaling Pathway (695207); Hemostasis Pathway (1120392); Matrix Metalloproteinases Pathway (198565); Platelet Activation, Signaling And Aggregation Pathway (1120402); Platelet Degranulation Pathway (1120414); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1120412)

96-Strip-Wells

445 USD