Rat IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) ELISA Kit | MyBioSource MBS2502844 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) ELISA Kit

catalog :

MBS2502844

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2502844

products type :

ELISA Kit

products full name :

Rat IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) ELISA Kit

products short name :

[IGFBP-4]

other names :

[insulin-like growth factor-binding protein 4; Insulin-like growth factor-binding protein 4; insulin-like growth factor-binding protein 4; IBP-4; IGF-binding protein 4; insulin-like growth factor binding protein 4]

products gene name :

[IGFBP-4]

other gene names :

[IGFBP4; IGFBP4; BP-4; IBP4; IGFBP-4; HT29-IGFBP; IBP4; IBP-4; IGF-binding protein 4; IGFBP-4]

uniprot entry name :

IBP4_HUMAN

reactivity :

Rat

sequence length :

258

specificity :

This kit recognizes Rat IGFBP-1 in samples. No significant cross-reactivity or interference between Rat IGFBP-1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IGFBP-1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IGFBP-1 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IGFBP-1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IGFBP-1. You can calculate the concentration of Rat IGFBP-1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

62243290

ncbi acc num :

NP_001543.2

ncbi gb acc num :

NM_001552.2

uniprot acc num :

P22692

ncbi mol weight :

17,635 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); Myometrial Relaxation And Contraction Pathways (198759); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908); Wnt Signaling Network Pathway (137962)

ncbi summary :

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]

uniprot summary :

IGFBP4: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: extracellular space; extracellular region. Molecular Function: insulin-like growth factor I binding; insulin-like growth factor II binding. Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; cell proliferation; regulation of insulin-like growth factor receptor signaling pathway; cellular protein metabolic process; positive regulation of MAPKKK cascade; regulation of cell growth; inflammatory response; signal transduction; skeletal development; DNA metabolic process

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590